Application of ZnO Nanoparticles for Improving the Thermal and pH Stability of Crude Cellulase Obtained from Aspergillus fumigatus AA001
نویسندگان
چکیده
Cellulases are the enzymes which are responsible for the hydrolysis of cellulosic biomass. In this study thermal and pH stability of crude cellulase has been investigated in the presence of zinc oxide (ZnO) nanoparticles. We synthesized ZnO nanoparticle by sol-gel method and characterized through various techniques including, X-ray Diffraction, ultraviolet-visible spectroscope, field emission scanning electron microscope and high resolution scanning electron microscope. The crude thermostable cellulase has been obtained from the Aspergillus fumigatus AA001 and treated with ZnO nanoparticle which shows thermal stability at 65°C up to 10 h whereas it showed pH stability in the alkaline pH range and retained its 53% of relative activity at pH 10.5. These findings may be promising in the area of biofuels production.
منابع مشابه
Crude cellulase from oil palm empty fruit bunch by Trichoderma asperellum UPM1 and Aspergillus fumigatus UPM2 for fermentable sugars production.
Cellulase is an enzyme that converts the polymer structure of polysaccharides into fermentable sugars. The high market demand for this enzyme together with the variety of applications in the industry has brought the research on cellulase into focus. In this study, crude cellulase was produced from oil palm empty fruit bunch (OPEFB) pretreated with 2% NaOH with autoclave, which was composed of 5...
متن کاملProduction of Xylanases and Cellulases by Aspergillus Fumigatus Ms16 Using Crude Lignocellulosic Substrates
Xylanolytic and cellulolytic potential of a soil isolate, Aspergillus fumigatus (MS16) was studied by growing it on a variety of lignocellulosics, purified cellulose and xylan supplemented media. It was noted that carboxymethyl cellulose, salicin and xylan induce the production of endoglucanase, β-glucosidase and xylanase, respectively. The study revealed that Aspergillus fumigatus (MS16) co-se...
متن کاملOptimization of Antibacterial Compounds Production by Aspergillus fumigatus Isolated from Sudanese Indigenous Soil
The purposes of the present study were to screen the ability of Aspergillus fumigatus to produce antibacterial compounds using different growth parameters namely, pH, temperature, agitation and time of fermentation and investigate the effect of the yield against bacterial isolates. A. fumigatus was isolated from arable lands in Sudan and identified macroscopically and microscopically. The pure ...
متن کاملInvestigation of Culture Conditions for Biosynthesis of Silver Nanoparticles Using Aspergillus fumigatus
In this study, silver nanoparticles were synthesized using the fungus, Aspergillus fumigatus. The effects of three independent variables including glucose content of culture media, initial pH and initial spore concentration on biosynthesis of silver nanoparticles were investigated. These variables affect cell morphology, cell mass, size and morphology of silver nanoparticles and degree of silv...
متن کاملProduction of Fermentable Sugars from Oil Palm Empty Fruit Bunch Using Crude Cellulase Cocktails with Trichoderma Asperellum Upm1 and Aspergillus Fumigatus Upm2 for Bioethanol Production
Utilization of oil palm empty fruit bunch (OPEFB) for bioethanol production with crude cellulase cocktails from locally isolated fungi was studied. Enzymatic saccharification of alkaline pretreated OPEFB was done using different cellulase enzyme preparations. Crude cellulase cocktails from Trichoderma asperellum UPM1 and Aspergillus fumigatus UPM2 produced 8.37 g/L reducing sugars with 0.17 g/g...
متن کامل